studying the impact of environmental and demographic parameters
Previous works indicate that cholesterol metabolism is deregulated in several types of malignancies including leukemia. Different types of cancer cells are previously shown to accumulate intracellular cholesterol. However, the data on intracellular cholesterol levels in leukemia cells provide contradictory evidence. Various previous works indicate either increase, decrease or no difference in total cellular cholesterol levels between leukemia cells and healthy peripheral blood mononuclear cells (PBMCs). We are studying in detail the lipid-load in leukemia cells and the associated factors. Our previous work indicates that PBMCs from AML and ALL patients display significantly lower intracellular levels of total cholesterol in comparison to PBMCs from the healthy subjects. Consistent with the patient data ALL and AML cell lines also display significantly lower cellular levels of total cholesterol. We confirmed this observation using multiple methodological approaches. Both ALL and AML cell lines also display significantly lower levels of free cholesterol and cholesteryl ester contents in comparison to normal hematopoietic cells. We observed that more than 90% of the total cholesterol in leukemia cells as well as in normal PBMCs was present in the form of cholesteryl esters. It was also observed that the lower levels of cholesterol in leukemia cells are not affected by exogenous cholesterol availability. Currently, we are assessing the impact of demographic and dietetic factors on lipid deposition in leukemia cells.
Related publications from our research group
Usman H, Ameer F, Munir R, Iqbal A, Zaid M, Hasnain S, Scandiuzzi L, Zaidi N: Leukemia cells display lower levels of intracellular cholesterol irrespective of the exogenous cholesterol availability. Clin Chim Acta 2016, 457:12-17
Regulation of plasma and cellular cholesterol levels in acute myeloid and lymphocytic leukemia
Thesis submitted in 2019
Factors affecting lipid and lipidomic profile of malignant and non-malignant hematopoietic cells.
Work in progress
Cholesterol Homeostasis in Acute Leukaemia Patients: Studying the Intra- and Extracellular Cholesterol Content
Lipid homeostasis in peripheral blood mononuclear cells